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dnase i solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dnase i solution
    Dnase I Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnase+i+solution/Deoxyribonuclease+I/pmc12830098-122-28-31
    Average 99 stars, based on 1 article reviews
    dnase i solution - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    TUNEL Assay:

    Article Title: GPER1 reduces skin inflammation by inhibiting keratinocyte proliferation
    Article Snippet: Samples were then washed twice for 5 min in PBST and incubated with 50 μL fresh TUNEL reaction mixture, consisting of 5 μL of enzyme solution mixed with 45 μL of labeling solution (In Situ Cell Death Detection Kit, Fluorescein, Roche), at 37 °C for 1 h. Finally, they were washed five times with PBST for 5 min each. .. As a TUNEL positive control, 2-dpf zebrafish embryos treated with DNAse I Solution (ThermoFisher Scientific #89836, 20 U/ml) in DNAse I buffer, incubated at room temperature for 10 min were used. .. Images were acquired on an epifluorescence LEICA MZ16FA stereomicroscope set up with green and red fluorescent filters, and the number of apoptotic cells was determined by counting them in blind samples.

    Article Title: GPER1 reduces skin inflammation by inhibiting keratinocyte proliferation.
    Article Snippet: Samples were then washed twice for 5 minutes in PBST and incubated with 50 μL fresh TUNEL reaction mixture, consisting of 5 μL of enzyme solution mixed with 45 μL of labeling solution (In Situ Cell Death Detection Kit, Fluorescein, Roche), at 37 °C for 1 h. Finally, they were washed 5 times with PBST for 5 min each. .. As a TUNEL positive control, 2-dpf zebrafish embryos treated with DNAse I Solution (ThermoFisher Scientific #89836, 20 U/ml) in DNAse I buffer incubated at room temperature for 10 min was used. .. Images were acquired on an epifluorescence LEICA MZ16FA stereomicroscope set up with green and red fluorescent filters and the number of apoptotic cells was determined by counting them in blind samples.

    Positive Control:

    Article Title: GPER1 reduces skin inflammation by inhibiting keratinocyte proliferation
    Article Snippet: Samples were then washed twice for 5 min in PBST and incubated with 50 μL fresh TUNEL reaction mixture, consisting of 5 μL of enzyme solution mixed with 45 μL of labeling solution (In Situ Cell Death Detection Kit, Fluorescein, Roche), at 37 °C for 1 h. Finally, they were washed five times with PBST for 5 min each. .. As a TUNEL positive control, 2-dpf zebrafish embryos treated with DNAse I Solution (ThermoFisher Scientific #89836, 20 U/ml) in DNAse I buffer, incubated at room temperature for 10 min were used. .. Images were acquired on an epifluorescence LEICA MZ16FA stereomicroscope set up with green and red fluorescent filters, and the number of apoptotic cells was determined by counting them in blind samples.

    Article Title: GPER1 reduces skin inflammation by inhibiting keratinocyte proliferation.
    Article Snippet: Samples were then washed twice for 5 minutes in PBST and incubated with 50 μL fresh TUNEL reaction mixture, consisting of 5 μL of enzyme solution mixed with 45 μL of labeling solution (In Situ Cell Death Detection Kit, Fluorescein, Roche), at 37 °C for 1 h. Finally, they were washed 5 times with PBST for 5 min each. .. As a TUNEL positive control, 2-dpf zebrafish embryos treated with DNAse I Solution (ThermoFisher Scientific #89836, 20 U/ml) in DNAse I buffer incubated at room temperature for 10 min was used. .. Images were acquired on an epifluorescence LEICA MZ16FA stereomicroscope set up with green and red fluorescent filters and the number of apoptotic cells was determined by counting them in blind samples.

    Incubation:

    Article Title: GPER1 reduces skin inflammation by inhibiting keratinocyte proliferation
    Article Snippet: Samples were then washed twice for 5 min in PBST and incubated with 50 μL fresh TUNEL reaction mixture, consisting of 5 μL of enzyme solution mixed with 45 μL of labeling solution (In Situ Cell Death Detection Kit, Fluorescein, Roche), at 37 °C for 1 h. Finally, they were washed five times with PBST for 5 min each. .. As a TUNEL positive control, 2-dpf zebrafish embryos treated with DNAse I Solution (ThermoFisher Scientific #89836, 20 U/ml) in DNAse I buffer, incubated at room temperature for 10 min were used. .. Images were acquired on an epifluorescence LEICA MZ16FA stereomicroscope set up with green and red fluorescent filters, and the number of apoptotic cells was determined by counting them in blind samples.

    Article Title: Engineering Clostridium thermocellum for production of 2,3-butanediol from cellulose
    Article Snippet: .. Cells were lysed by the addition of 1 μL of Ready-Lyse lysozyme solution (Epicenter, WI, USA) and incubated at room temperature for 20 min. Then, 1 μL of DNase I solution (Thermo Scientific, MA, USA) was added, and lysate was incubated for an additional 20 min at room temperature. ..

    Article Title: Zinc‐Containing Bioactive Glass Programs Macrophage Polarization through Extracellular Traps Regulation for Enhanced Diabetic Wound Healing
    Article Snippet: .. To evaluate the MET‐clearing effects of ZnBG and DNase I, high‐glucose‐treated cells were incubated with BG extract and DNase I solution (Thermo Scientific, 10 U/mL, as previously described by Weng et al. [ ]) for 12 h, followed by METs quantification. .. A laser Doppler imaging system (Perimed PSI HR model) was used to evaluate the wound perfusion status on day 15 post‐injury.

    Article Title: Bioinspired, Guanidinium, and Indole Modified Poly(glycidyl ether)s as Highly Efficient Vectors for Polyplex‐Mediated Gene Delivery
    Article Snippet: .. After the above described 10‐min incubation for polyplex assembly, 2 μL of DNase I solution (ready‐to‐use solution with 2.5 kunitzU per μL, containing 10 m m Tris‐HCl (pH 7.5), 10 m m CaCl 2 and 10 m m MgCl 2 ; Thermo Fisher Scientific, Darmstadt, Germany) was added, followed by 45 min of incubation at 37°C in a thermocycler (Biometra TOne, Analytik Jena, Jena, Germany). ..

    Article Title: GPER1 reduces skin inflammation by inhibiting keratinocyte proliferation.
    Article Snippet: Samples were then washed twice for 5 minutes in PBST and incubated with 50 μL fresh TUNEL reaction mixture, consisting of 5 μL of enzyme solution mixed with 45 μL of labeling solution (In Situ Cell Death Detection Kit, Fluorescein, Roche), at 37 °C for 1 h. Finally, they were washed 5 times with PBST for 5 min each. .. As a TUNEL positive control, 2-dpf zebrafish embryos treated with DNAse I Solution (ThermoFisher Scientific #89836, 20 U/ml) in DNAse I buffer incubated at room temperature for 10 min was used. .. Images were acquired on an epifluorescence LEICA MZ16FA stereomicroscope set up with green and red fluorescent filters and the number of apoptotic cells was determined by counting them in blind samples.

    Article Title: Bioinspired, Guanidinium, and Indole Modified Poly(glycidyl ether)s as Highly Efficient Vectors for Polyplex-Mediated Gene Delivery.
    Article Snippet: .. After the above described 10-min incubation for polyplex assembly, 2 μL of DNase I solution (ready-to-use solution with 2.5 kunitzU per μL, containing 10 m m Tris-HCl (pH 7.5), 10 m m CaCl2 and 10 m m MgCl2 ; Thermo Fisher Scientific, Darmstadt, Germany) was added, followed by 45 min of incubation at 37◦C in a thermocycler (Biometra TOne, Analytik Jena, Jena, Germany). ..

    Recombinant:

    Article Title: Mitochondrial cargo quality determines the paracrine effects of extracellular vesicles derived from vascular endothelial cells.
    Article Snippet: .. Annexin V conjugated to fluorescein isothiocyanate (FITC; A13199), annexin V conjugated to allophycocyanin (APC; A35110), MitoTrackerTM Green FM (M7514), MitoTrackerTM Red FM (M22425), human TNF-α recombinant protein (A42551), DNase I solution, RNasefree (PI89836), and Invitrogen CellLightTM Mitochondria-red fluorescent protein (RFP) BacMam 2.0 (C10601) were purchased from ThermoFisher Scientific. .. Human red blood cell (RBC)-derived EV (CBS3) were from Cellarcus Biosciences.



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